Melanoma skin cancer remains incurable, and targeting heat shock protein 90 (Hsp90) is a promising therapeutic approach. In this study, we investigated the effects of 17-aminogeldanamycin, a potent inhibitor of Hsp90, the nuclear factor-kappa B (NF-kB) activity in a patient-derived melanoma cell lines and NRASQ61R BRAFV600E.
We did a time-lapse microscopy and flow cytometry to monitor changes in cell meeting and survival. NF-kB activity is determined by Immunodetection of phospho-p65 and assessment of gene expression of NF-kB dependent by quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting and enzyme-linked immunosorbent assay (ELISA).
constitutive activity of p65 / NF-kB was evident in all melanoma cell lines. The difference in rates may be linked to genetic changes in CHUK, IL1B, MAP3K14, NFKBIE, RIPK1, and TLR4, while the difference in the level of gene transcripts-induced NF-kB revealed by PCR arrays that may arise from the contribution of other regulatory mechanisms. 17-Aminogeldanamycin markedly reduced levels of phospho-p65, but the level of total p65 protein is not changed, indicating that the 17-aminogeldanamycin inhibits activation of p65 / NF-kB.
This conclusion is supported by a significantly reduced expression of selected genes of NF-kB-dependent: cyclin D1 (CCND1), CXC motif chemokine ligand 8 (CXCL8), and vascular endothelial growth factor (VEGF), as shown in the level of transcript and protein, as well as the secretion of IL-8 and VEGF. Our studies show that 17-aminogeldanamycin can be used for efficient inhibition of NF-kB activity and a simultaneous decrease in the level of IL-8 and VEGF in melanoma extracellular environment.
17-Aminogeldanamycin Inhibits Constitutive Nuclear Factor-Kappa B (NF-κB) Activity in Patient-Derived Melanoma Cell Lines
Baicalin Inhibits Proliferation and inflammatory cytokine Cells Induced by Tumor Necrosis Factor α (TNF-α) in Human immortalized keratinocytes (HaCaT) Human keratinocytes by Inhibiting STAT3 / Nuclear Factor kappa B (NF-kB) Signaling Pathway.
BACKGROUND baicalin is isolated flavonoids from Scutellaria baicalensis roots and used in traditional Chinese medicine. Psoriasis is a persistent skin disease and chronic recurrent inflammation that is characterized by inflammation and increasing keratinocyte proliferation. This study aimed to investigate the effects of baicalin on HaCaT immortalized human keratinocytes in vitro and molecular mechanisms involved.
MATERIALS AND METHODS HaCaT keratinocytes cultured in increasing concentrations of baicalin at 6.25 pM, 12.5 pM, and 25 pM. Psoriasis established in vitro model using HaCaT cells were treated with tumor necrosis factor-alpha (TNF-alpha). MTT assay was used to asses cell viability and apoptosis.
Description: A sandwich ELISA for quantitative measurement of Human soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Rabbit soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Goat soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Porcine Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Porcine Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Porcine Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Porcine soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat soluble urokinase plasminogen activator receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Bovine Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates or other biological fluids.
Human Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates or other biological fluids.
Rat Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma or other biological fluids.
Rat Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Rat Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Monkey Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Monkey Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response.
Thrombin Receptor Activator for Peptide 5 (TRAP-5)
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response.
Thrombin Receptor Activator for Peptide 5 (TRAP-5)
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response.
Description: A sandwich ELISA for quantitative measurement of Monkey soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Monkey soluble urokinase plasminogen activator receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human soluble Urokinase- type Plasminogen Activator Receptor, su
Description: A sandwich ELISA for quantitative measurement of Monkey Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Urokinase type Plasminogen Activator Receptor ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey Urokinase type Plasminogen Activator Receptor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Plasminogen Activator, Urokinase Receptor (uPAR) in serum, plasma, tissue homogenates and other biological fluids.
Human Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Plasminogen Activator, Urokinase Receptor (uPAR) in serum, plasma, tissue homogenates and other biological fluids.
Human Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Plasminogen Activator, Urokinase Receptor (uPAR) in serum, plasma, tissue homogenates and other biological fluids.
Human Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Plasminogen Activator, Urokinase Receptor (uPAR) in serum, plasma, tissue homogenates and other biological fluids.
Human Plasminogen Activator, Urokinase Receptor (uPAR) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator, Urokinase Receptor (uPAR) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator, Urokinase Receptor (uPAR) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator, Urokinase Receptor (uPAR) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Plasminogen Activator, Urokinase Receptor (uPAR) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Plasminogen Activator, Urokinase Receptor (uPAR) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
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Western blot was used to measure the expression of Bcl-2, Bax, pro-caspase-3 and cleaved caspase-3, and enzyme-linked immunosorbent assay (ELISA) is performed to detect inflammatory cytokines. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the level of STAT3 and p65 mRNA. RESULTS baicalin reduce cell viability and induces apoptosis of HaCaT human keratinocytes in a dose-dependent manner.
The increase in cell survival and expression of inflammatory cytokines by HaCaT cells induced by TNF-alpha was significantly inhibited by baicalin. Baicalin significantly inhibited the activation of STAT3 / NF-kappaB in HaCaT cells stimulated by TNF-alpha. CONCLUSION baicalin inhibits proliferation and inflammatory cytokine expression in HaCaT immortalized human keratinocytes in vitro by inhibition of STAT3 / NF-kappaB signaling pathway.
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