Melanoma skin cancer remains incurable, and targeting heat shock protein 90 (Hsp90) is a promising therapeutic approach. In this study, we investigated the effects of 17-aminogeldanamycin, a potent inhibitor of Hsp90, the nuclear factor-kappa B (NF-kB) activity in a patient-derived melanoma cell lines and NRASQ61R BRAFV600E.
We did a time-lapse microscopy and flow cytometry to monitor changes in cell meeting and survival. NF-kB activity is determined by Immunodetection of phospho-p65 and assessment of gene expression of NF-kB dependent by quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting and enzyme-linked immunosorbent assay (ELISA).
constitutive activity of p65 / NF-kB was evident in all melanoma cell lines. The difference in rates may be linked to genetic changes in CHUK, IL1B, MAP3K14, NFKBIE, RIPK1, and TLR4, while the difference in the level of gene transcripts-induced NF-kB revealed by PCR arrays that may arise from the contribution of other regulatory mechanisms. 17-Aminogeldanamycin markedly reduced levels of phospho-p65, but the level of total p65 protein is not changed, indicating that the 17-aminogeldanamycin inhibits activation of p65 / NF-kB.
This conclusion is supported by a significantly reduced expression of selected genes of NF-kB-dependent: cyclin D1 (CCND1), CXC motif chemokine ligand 8 (CXCL8), and vascular endothelial growth factor (VEGF), as shown in the level of transcript and protein, as well as the secretion of IL-8 and VEGF. Our studies show that 17-aminogeldanamycin can be used for efficient inhibition of NF-kB activity and a simultaneous decrease in the level of IL-8 and VEGF in melanoma extracellular environment.
17-Aminogeldanamycin Inhibits Constitutive Nuclear Factor-Kappa B (NF-κB) Activity in Patient-Derived Melanoma Cell Lines
Baicalin Inhibits Proliferation and inflammatory cytokine Cells Induced by Tumor Necrosis Factor α (TNF-α) in Human immortalized keratinocytes (HaCaT) Human keratinocytes by Inhibiting STAT3 / Nuclear Factor kappa B (NF-kB) Signaling Pathway.
BACKGROUND baicalin is isolated flavonoids from Scutellaria baicalensis roots and used in traditional Chinese medicine. Psoriasis is a persistent skin disease and chronic recurrent inflammation that is characterized by inflammation and increasing keratinocyte proliferation. This study aimed to investigate the effects of baicalin on HaCaT immortalized human keratinocytes in vitro and molecular mechanisms involved.
MATERIALS AND METHODS HaCaT keratinocytes cultured in increasing concentrations of baicalin at 6.25 pM, 12.5 pM, and 25 pM. Psoriasis established in vitro model using HaCaT cells were treated with tumor necrosis factor-alpha (TNF-alpha). MTT assay was used to asses cell viability and apoptosis.
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response.
Thrombin Receptor Activator for Peptide 5 (TRAP-5)
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response.
Thrombin Receptor Activator for Peptide 5 (TRAP-5)
Description: Thrombin Receptor Activator for Peptide 5 (TRAP-5),(C30H50N8O7), a peptide with the sequence NH2-Ser-Phe-Leu-Leu-Arg-COOH, MW= 634.76. coagulation factor II (thrombin) receptor is a G protein-coupled receptor involved in the regulation of thrombotic response.
Thrombin Receptor Activator for Peptide 5 (TRAP-5)
Description: Recombinant Human Receptor Activator Of Nuclear Factor Kappa B expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells
Active Receptor Activator Of Nuclear Factor Kappa B (RANk)
Description: Recombinant Mouse Receptor Activator Of Nuclear Factor Kappa B Ligand expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells
Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Description: Recombinant Mouse Receptor Activator Of Nuclear Factor Kappa B Ligand expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells
Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Description: Recombinant Rat Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL) expressed in: Available from E.coli, Yeast, Baculovirus and Mammalian cells
Active Receptor Activator Of Nuclear Factor Kappa B Ligand (RANkL)
Description: A competitive ELISA for quantitative measurement of Rat Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Rat Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Canine Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Dog receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Canine receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rat receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Porcine Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Porcine receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Porcine receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Pig receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Porcine receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Goat Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Goat receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Goat receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse receptor activator of nuclear factor kappa B ELISA Kit
Description: A competitive ELISA for quantitative measurement of Mouse Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Mouse Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Mouse receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Human Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Human receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rat soluble Receptor Activator of Nuclear Factor- kB Ligand, sRA
Description: A sandwich ELISA for quantitative measurement of Rabbit receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Rabbit receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Rabbit Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Rabbit Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey Receptor Activator of Nuclear Factor Kb Ligand ELISA kit
Description: A competitive ELISA for quantitative measurement of Monkey Receptor Activator of Nuclear Factor Kb Ligand in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Human soluble receptor activator of nuclear factor- kB ligand, s
Description: A sandwich ELISA for quantitative measurement of Monkey receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Monkey receptor activator of nuclear factor-kB (RANK) ELISA kit
Description: A sandwich ELISA for quantitative measurement of Monkey receptor activator of nuclear factor-kB (RANK) in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Mouse soluble receptor activator of nuclear factor- kB ligand, s
Description: Quantitative sandwich ELISA for measuring Human Steroid receptor RNA activator 1 (SRA1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Steroid receptor RNA activator 1 (SRA1)
Description: Quantitative sandwich ELISA for measuring Human Steroid receptor RNA activator 1 (SRA1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Human Steroid receptor RNA activator 1 (SRA1)
Description: Quantitative sandwich ELISA for measuring Human Steroid receptor RNA activator 1 (SRA1) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Monkey Plasminogen Activator, Urokinase Receptor (uPAR) Protein
Western blot was used to measure the expression of Bcl-2, Bax, pro-caspase-3 and cleaved caspase-3, and enzyme-linked immunosorbent assay (ELISA) is performed to detect inflammatory cytokines. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to detect the level of STAT3 and p65 mRNA. RESULTS baicalin reduce cell viability and induces apoptosis of HaCaT human keratinocytes in a dose-dependent manner.
The increase in cell survival and expression of inflammatory cytokines by HaCaT cells induced by TNF-alpha was significantly inhibited by baicalin. Baicalin significantly inhibited the activation of STAT3 / NF-kappaB in HaCaT cells stimulated by TNF-alpha. CONCLUSION baicalin inhibits proliferation and inflammatory cytokine expression in HaCaT immortalized human keratinocytes in vitro by inhibition of STAT3 / NF-kappaB signaling pathway.
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