Baicalin Inhibits Cell Proliferation and Inflammatory Cytokines Induced by Tumor Necrosis Factor α (TNF-α) in Human Immortalized Keratinocytes (HaCaT) Human Keratinocytes by Inhibiting the STAT3/Nuclear Factor kappa B (NF-κB) Signaling Pathway.

Baicalin Inhibits Cell Proliferation and Inflammatory Cytokines Induced by Tumor Necrosis Factor α (TNF-α) in Human Immortalized Keratinocytes (HaCaT) Human Keratinocytes by Inhibiting the STAT3/Nuclear Factor kappa B (NF-κB) Signaling Pathway.

BACKGROUND quercetin (Que) reported to induce apoptosis of lung cancer cells. Src is closely related to the development of lung cancer is non-small cell (NSCLC) and can be affected by Que in macrophages. In this study, the interaction between Que and Src signaling in NSCLC cell NSCLC explored to explain the anti-function Que.

MATERIALS AND METHODS HCC827 NSCLC cell lines were subjected to administration of Que at different concentrations. Que influence on tumor cell proliferation was detected using MTT and colony formation test. Then the effect on the migration and invasion ability was assessed using the initial and Transwell test. At the molecular level, changes in Src / Fn14 / NF-kappaB signaling is determined using western blotting tests.

The role of Src in Que function further explored by inducing the expression of Src gene in NSCLC cells prior to administration Que. The test results were verified in vitro using a mouse model of NSCLC. RESULTS Que inhibits the proliferation and anchorage-independent cell growth in NSCLC. Additionally, Que delayed in closing a gap in the level of initial tests and a decrease in the number of cells in the membrane-penetrating Transwell test. At the molecular level, Que suppressed the expression of Src, which then inhibit Fn14 / NF-kappaB signaling.

In in vivo tests, Que inhibits the growth of solid tumors. After the excess of Src in NSCLC cells, the effect of anti-NSCLC NSCLC Que blocked by inducing the proliferation and metastasis, and by activating Fn14 / NF-kappaB signaling. In addition, the induced levels of Src promoted the growth and metastatic potential of solid tumors in mice. CONCLUSION Que anti-NSCLC given effect by inhibiting Src-mediated Fn14 / NF-kappaB pathway both in vitro and in vivo.

Baicalin Inhibits Cell Proliferation and Inflammatory Cytokines Induced by Tumor Necrosis Factor α (TNF-α) in Human Immortalized Keratinocytes (HaCaT) Human Keratinocytes by Inhibiting the STAT3/Nuclear Factor kappa B (NF-κB) Signaling Pathway.
Baicalin Inhibits Cell Proliferation and Inflammatory Cytokines Induced by Tumor Necrosis Factor α (TNF-α) in Human Immortalized Keratinocytes (HaCaT) Human Keratinocytes by Inhibiting the STAT3/Nuclear Factor kappa B (NF-κB) Signaling Pathway.

Osteoarthritis Prevented in Mice by Verbascoside via Nuclear Factor kappa B (NF-kB) Downregulation Pathway.

BACKGROUND Cartilage degeneration for osteoarthritis (OA), the most adversely affects the quality of life by inhibiting the movement. This study investigated the verbascoside role in the protection of cartilage degeneration caused by osteoarthritis. MATERIALS AND METHODS enzyme-linked immunosorbent assay (ELISA) and western blot test is used to determine the secretion of inflammatory cytokines in serum and cartilage tissue, respectively.

RESULTS Treatment of the mice with excess verbascoside OA inhibiting interleukin (IL) -6, tumor necrosis factor (TNF) -alpha, and IL-1SS in serum and cartilage tissue.

P2X7R expression and matrix metalloproteinase (MMP) -13 is much higher in mice induced by OA. However, the administration reversed verbascoside OA-induced upregulation of P2X7R and MMP-13 expression in cartilage tissue. OA-mediated increase in substance P (SP) and prostaglandin E2 (PGE2) expression was also reduced in cartilage tissue by verbascoside treatment. Western blot assay revealed that treatment verbascoside IkappaBalpha markedly decreased activation of NF-kappaB and p65 in mice OA.

CONCLUSION So, verbascoside inhibits the secretion of inflammatory cytokines in rats OA by targeting P2X7R expression, the production of matrix metalloproteinases, PGE2 and downregulation of NF-kappaB signaling pathway. Therefore, verbascoside can be used as an effective agent for the treatment of osteoarthritis.

The Onco-suppressor p53 is a transcription factor that regulates a broad spectrum of genes involved in various cellular functions including apoptosis, cell cycle arrest, senescence, autophagy, DNA repair and angiogenesis. p53 and NF-kB generally has the opposite effect on the cancer cells.

Kappa Light Chain (Kappa-IgLC) Antibody

abx412052-1ml 1 ml
EUR 526.8

Kappa Light Chain (Kappa-IgLC) Antibody

abx413125-1mg 1 mg
EUR 994.8

Kappa Light Chain (Kappa-IgLC) Antibody

abx415783-1mg 1 mg
EUR 526.8

Kappa Light Chain (Kappa-IgLC) Antibody

abx415786-05mg 0.5 mg
EUR 477.6

Kappa Light Chain (Kappa-IgLC) Antibody

abx415797-1mg 1 mg
EUR 594

Kappa Light Chain (Kappa-IgLC) Antibody

abx415799-05mg 0.5 mg
EUR 477.6

Kappa Light Chain (Kappa-IgLC) Antibody

abx411144-200g 200 µg
EUR 437.5

Kappa Light Chain (Kappa-IgLC) Antibody

abx411422-100tests 100 tests
EUR 550

Kappa Light Chain (Kappa-IgLC) Antibody

abx412052-250g 250 µg
EUR 325

Kappa Light Chain (Kappa-IgLC) Antibody

abx413125-200l 200 µl
EUR 712.5

Kappa Light Chain (Kappa-IgLC) Antibody

abx415783-200l 200 µl
EUR 325

Kappa Light Chain (Kappa-IgLC) Antibody

abx415786-200l 200 µl
EUR 400

Kappa Light Chain (Kappa-IgLC) Antibody

abx415797-200l 200 µl
EUR 450

Kappa Light Chain (Kappa-IgLC) Antibody

abx415799-200l 200 µl
EUR 300

immunoglobulin Kappa (immunoglobulin kappa) Antibody

abx234295-100ug 100 ug
EUR 610.8

Kappa Light Chain (Kappa-IgLC) Antibody (AP)

abx411146-1ml 1 ml
EUR 678

Kappa Light Chain (Kappa-IgLC) Antibody (AP)

abx411216-1ml 1 ml
EUR 678

Kappa Light Chain (Kappa-IgLC) Antibody (PE)

abx411219-250g 250 µg
EUR 650

Kappa Light Chain (Kappa-IgLC) Antibody (PE)

abx415789-200l 200 µl
EUR 525

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx411147-1ml 1 ml
EUR 594

Kappa Light Chain (Kappa-IgLC) Antibody (RPE)

abx411150-025mg 0.25 mg
EUR 760.8

Kappa Light Chain (Kappa-IgLC) Antibody (RPE)

abx411219-05mg 0.5 mg
EUR 910.8

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx412923-05mg 0.5 mg
EUR 861.6

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx413129-01mg 0.1 mg
EUR 594

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx414986-05mg 0.5 mg
EUR 861.6

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx415785-1ml 1 ml
EUR 594

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx415788-1ml 1 ml
EUR 594

Kappa Light Chain (Kappa-IgLC) Antibody (RPE)

abx415789-05ml 0.5 ml
EUR 760.8

Kappa Light Chain (Kappa-IgLC) Antibody (ALP)

abx411146-200g 200 µg
EUR 462.5

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx411147-200g 200 µg
EUR 425

Kappa Light Chain (Kappa-IgLC) Antibody (ALP)

abx411216-25g 25 µg
EUR 450

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx412923-200l 200 µl
EUR 662.5

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx413129-200l 200 µl
EUR 375

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx414986-200l 200 µl
EUR 600

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx415785-200l 200 µl
EUR 387.5

Kappa Light Chain (Kappa-IgLC) Antibody (HRP)

abx415788-200l 200 µl
EUR 387.5

Kappa Light Chain (Kappa-IgLC) Antibody (FITC)

abx411143-05mg 0.5 mg
EUR 526.8

Kappa Light Chain (Kappa-IgLC) Antibody (FITC)

abx415784-1mg 1 mg
EUR 560.4

Human Kappa Light Chain (Kappa-IgLC) Protein

abx670245-1mg 1 mg
EUR 661.2

Human Kappa Light Chain (Kappa-IgLC) Protein

abx670246-1mg 1 mg
EUR 777.6

Human Kappa Light Chain (Kappa-IgLC) Protein

abx670245-100g 100 µg
EUR 462.5

Human Kappa Light Chain (Kappa-IgLC) Protein

abx670246-100g 100 µg
EUR 562.5

Kappa Light Chain (Kappa-IgLC) Antibody (FITC)

abx411143-200g 200 µg
EUR 325

Kappa Light Chain (Kappa-IgLC) Antibody (FITC)

abx415784-200l 200 µl
EUR 362.5

Ig Kappa

ant-202 0.5mg
EUR 275
Description: Mouse Anti Human Ig Kappa Light Chain

Kappa Light Chain (Kappa-IgLC) Antibody (TRITC)

abx411145-05mg 0.5 mg
EUR 744

Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)

abx411149-1mg 1 mg
EUR 727.2

Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)

abx411218-1mg 1 mg
EUR 594

Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)

abx415787-05mg 0.5 mg
EUR 560.4

Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)

abx411149-200g 200 µg
EUR 487.5

Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)

abx411218-50g 50 µg
EUR 387.5

Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)

abx415787-200l 200 µl
EUR 362.5

IgE kappa

GWB-CA2911 1 ml Ask for price

Mouse Immunoglobulin M Kappa (IgM Kappa) Protein

abx670294-1mg 1 mg
EUR 760.8

Mouse Immunoglobulin E Kappa (IgE Kappa) Protein

abx670299-05mg 0.5 mg
EUR 727.2

OPRA03561-1MG - IgM Kappa (kappa) myeloma protein

OPRA03561-1MG 1mg
EUR 249

OPRA03569-1MG - IgA Kappa (kappa) myeloma protein

OPRA03569-1MG 1mg
EUR 249

Mouse Immunoglobulin M Kappa (IgM Kappa) Protein

abx670294-100g 100 µg
EUR 637.5

Mouse Immunoglobulin E Kappa (IgE Kappa) Protein

abx670299-100g 100 µg
EUR 512.5

Human Immunoglobulin G1 Kappa (IgG1 Kappa) Protein

abx670165-1mg 1 mg
EUR 560.4

Human Immunoglobulin G2 Kappa (IgG2 Kappa) Protein

abx670166-1mg 1 mg
EUR 627.6

Mouse Immunoglobulin G1 Kappa (IgG1 Kappa) Protein

abx670291-1mg 1 mg
EUR 760.8

OPRA03544-1MG - IgG1 Kappa (kappa) myeloma protein

OPRA03544-1MG 1mg
EUR 249

Mouse Immunoglobulin G1 Kappa (IgG1 Kappa) Protein

abx670291-100g 100 µg
EUR 700

KAPPA/CD19

KAPPAF219PE1-50T 50 test
EUR 420.36

KAPPA/CD19

KF319PE4-50T 50 test
EUR 559.2

OPRA03556-1MG - IgG2b Kappa (kappa) myeloma protein

OPRA03556-1MG 1mg
EUR 249

OPRA03558-1MG - IgG2a Kappa (kappa) myeloma protein

OPRA03558-1MG 1mg
EUR 249

Mouse Immunoglobulin G2a Kappa (IgG2a Kappa) Protein

abx670292-1mg 1 mg
EUR 760.8

Mouse Immunoglobulin G2b Kappa (IgG2b Kappa) Protein

abx670293-1mg 1 mg
EUR 760.8

Mouse Immunoglobulin G2a Kappa (IgG2a Kappa) Protein

abx670292-100g 100 µg
EUR 612.5

Mouse Immunoglobulin G2b Kappa (IgG2b Kappa) Protein

abx670293-50g 50 µg
EUR 587.5

Kappa (NFKB1)

MO47031 100 ul
EUR 418.8

Human Kappa Light Chain (Kappa-IgLC) ELISA Kit

abx055741-96tests 96 tests
EUR 801.6

Casein kappa

E8ER1905-46 100ul
EUR 275
Description: Available in various conjugation types.

LAMBDA/KAPPA

LF2KPE3-50T 50 test
EUR 738.6

KAPPA/LAMBDA

KAPPAF2LAMBDAPE-50T 50 test
EUR 496.8

Kappa Light Chain (Kappa-IgLC) Antibody (Texas Red)

abx411148-05mg 0.5 mg
EUR 744

Kappa Light Chain (Kappa-IgLC) Antibody (Texas Red)

abx411217-1mg 1 mg
EUR 560.4

Kappa Light Chain (Kappa-IgLC) Antibody (Texas Red)

abx411217-500g 500 µg
EUR 362.5

Kappa-casein

AP81249 1mg
EUR 2640

Kappa-casein

AP81342 1mg
EUR 2640

Kappa-casein

AP81343 1mg
EUR 2640

Kappa-casein

AP81456 1mg
EUR 2640

Kappa-casein

AP81505 1mg
EUR 2640

Kappa Antibody

P1045-01m 0.1m
EUR 163.2

Kappa Antibody

P1045-1ml 1ml
EUR 337.2

Immunoglobulin Kappa Light Chain (Ig kappa LC) Antibody

abx137077-01mg 0.1 mg
EUR 487.5

Carrageenan, Kappa

40300044-1 100 g
EUR 35.99

Carrageenan, Kappa

40300044-2 500 g
EUR 146.28

kappa-Bifenthrin

T24244-10mg 10mg Ask for price
Description: kappa-Bifenthrin

kappa-Bifenthrin

T24244-1g 1g Ask for price
Description: kappa-Bifenthrin

kappa-Bifenthrin

T24244-1mg 1mg Ask for price
Description: kappa-Bifenthrin

kappa-Bifenthrin

T24244-50mg 50mg Ask for price
Description: kappa-Bifenthrin

kappa-Bifenthrin

T24244-5mg 5mg Ask for price
Description: kappa-Bifenthrin

Kappa casein ELISA Kit| Bovine Kappa casein ELISA Kit

EF011008 96 Tests
EUR 826.8

kappa-Carrageenan

GC6839-1 1
EUR 193.7

kappa-Carrageenan

GC6839-100 100
EUR 31.7

kappa-Carrageenan

GC6839-100G 100 g
EUR 74.4

kappa-Carrageenan

GC6839-1KG 1 kg
EUR 270

kappa-Carrageenan

GC6839-25 25
EUR 13.9

kappa-Carrageenan

GC6839-250 250
EUR 63.3

kappa-Carrageenan

GC6839-250G 250 g
EUR 112.8

kappa-Carrageenan

GC6839-25G 25 g
EUR 51.6

kappa-Carrageenan

GC6839-500 500
EUR 110.7

kappa-Carrageenan

GC6839-500G 500 g
EUR 170.4

Kappa/Lambda Light Chain (Kappa/Lambda-IgLC) Antibody (HRP)

abx412924-05mg 0.5 mg
EUR 1028.4

Kappa/Lambda Light Chain (Kappa/Lambda-IgLC) Antibody (HRP)

abx412924-200l 200 µl
EUR 737.5

K (kappa) chain

RP-4020-01 0.5 ml
EUR 136.15

K (kappa) chain

RP-4020-02 1.0 ml
EUR 198.8

Mouse IgM Kappa

31R-1091 100 ug
EUR 151
Description: Purified Mouse IgM Kappa Isotype Control

Mouse IgA Kappa

GWB-CB81B7 1 mg Ask for price

Kappa ELISA Kit

80722 96
EUR 445

Human IgG2 kappa

GWB-E6FDE0 1 mg Ask for price

Kappa Light Chain

10R-8329 100 ug
EUR 218
Description: Mouse monoclonal Kappa Light Chain antibody

Kappa Light Chain

A00064 6 ml
EUR 129.6

Kappa Light Chain

A00064.0025 25 ml
EUR 302.4

Kappa Light Chain

A20064 2 ml
EUR 94.8

IgA kappa Protein

abx060686-1mg 1 mg
EUR 777.6

KAPPA Antibody (PE)

abx200537-100tests 100 tests
EUR 1011.6

KAPPA Antibody (PE)

abx200539-100tests 100 tests
EUR 727.2

IgE, kappa Protein

GWB-T00005 1mg Ask for price

Mouse IgG2a kappa

GWB-741A84 1 mg Ask for price

Human Kappa ELISA

GWB-HKAPPA 96 well Ask for price

KAPPA/LAMBDA/CD19

KAPPAF2LAMBDAPE19PC2-50T 50 test
EUR 941.4

Human Kappa ELISA

KT-522 96 tests
EUR 506

Kappa Light Chain

1107NF-V7080 0.5/ml
EUR 180

Kappa Light Chain

V1056 0.2mg
EUR 180

Human Kappa Light Chain mouse monoclonal antibody, clone kappa-117, Purified

AM26735PU-N 100 µg Ask for price

PTP kappa Antibody

E38PA1976 100ul
EUR 225
Description: Available in various conjugation types.

PTP kappa antibody

70R-50295 100 ul
EUR 242
Description: Purified Polyclonal PTP kappa antibody

KAPPA Antibody (APC)

abx201104-100tests 100 tests
EUR 777.6

DGK kappa Antibody

20-abx133186
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 30 ul

PTP kappa Antibody

20-abx008797
  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 30 ul

IgG4, kappa Protein

GWB-T00749 1mg Ask for price

RBPJ kappa antibody

70R-13485 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal RBPJ kappa antibody

KAPPA Antibody (FITC)

abx200536-100tests 100 tests
EUR 828

KAPPA Antibody (FITC)

abx200538-100tests 100 tests
EUR 727.2

kappa chain Antibody

GWB-102790 0.25 mg Ask for price

Kappa actin antibody

70R-51228 100 ul
EUR 242
Description: Purified Polyclonal Kappa actin antibody

kappa actin Antibody

20-abx007912
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  • Ask for price
  • Ask for price
  • 100 ul
  • 200 ul
  • 30 ul

Kappa Control Slides

ICS2027-25 25 Slides
EUR 137.81

Kappa Opioid Receptor

MO15098 100 ug
EUR 459.6

Kappa Opioid Receptor

RA10103T 50 ul
EUR 212.4

Kappa Opioid Receptor

RA10103T-B each
EUR 87.5

NF kappa B p65

PC-372 Polyclonal Ask for price

Kappa-actin Antibody

E38PA2909 100ul
EUR 225
Description: Available in various conjugation types.

PE human IgG1 Kappa

E16FHCP001K-100U 0.1 mg
EUR 476.67
Description: Available in various conjugation types.

NE human IgG1 Kappa

E16FHCY001K-500U 0.5 mg
EUR 520
Description: Available in various conjugation types.

Kappa Antibody, Concentrate

MAB363C 0.2ml
EUR 425

Kappa-Theraphotoxin / SGTx1

023-99 100 μg
EUR 461.16

Kappa Light Chain (HRP)

10R-8330 100 ug
EUR 233
Description: Mouse monoclonal Kappa Light Chain antibody (HRP)

NFR kappa B Antibody

20-abx217161
  • Ask for price
  • Ask for price
  • 100 ug
  • 50 ug

kappa Actin Rabbit pAb

E2342461 100μl
EUR 225
Description: Available in various conjugation types.

immunoglobulin kappa Antibody

abx234295-100g 100 µg
EUR 350

Human IgE Kappa protein

31C-CP1035K 100 ug
EUR 347
Description: Purified Human IgE Kappa protein

Human IgM Kappa protein

31R-AI088S 1 mg
EUR 102
Description: Purified Human IgM Kappa protein

Human IgE Myeloma, Kappa

31-1010 100 ug
EUR 460
Description: Human IgE Myeloma, Kappa Protein

Human Kappa Light Chain

40-KG05 1 L Ask for price
Description: Human Kappa Light Chain antibody

Kappa Antibody (IHC Gold)

E36PA610 100ul
EUR 225
Description: Available in various conjugation types.

Mouse IgM kappa Protein

abx060693-1mg 1 mg
EUR 794.4

Mouse IgM, kappa Protein

abx160025-1mg 1 mg
EUR 844.8

kappa chain Antibody (PE)

GWB-AAF16D 0.05 mg Ask for price

IgG kappa chain antibody

10-1935 200 ul
EUR 651.6
Description: Mouse monoclonal IgG kappa chain antibody

Purified human IgA Kappa

E16FHCU011K-500U 0.5 mg
EUR 433.33
Description: Available in various conjugation types.

Human Myeloma IgA2 kappa

GWB-50D1F7 1 mg Ask for price

Mouse IgG1 kappa Protein

abx060692-1mg 1 mg
EUR 627.6

Human IgG2 kappa Protein

abx060993-1mg 1 mg
EUR 460.8

Human IgG4 kappa Protein

abx060995-1mg 1 mg
EUR 678

Human Myeloma IgA1 kappa

GWB-81CAAB 5 mg Ask for price

Anti-NF kappa antibody

STJ94454 200 µl
EUR 236.4
Description: Rabbit polyclonal to NFRkappaB.

Purified human IgG1 Kappa

E16FHCU001K-500U 0.5 mg
EUR 433.33
Description: Available in various conjugation types.

Mouse IgG2a, kappa Protein

abx061466-1mg 1 mg
EUR 861.6

kappa chain Antibody (FITC)

GWB-89AA8E 0.1 mg Ask for price

Anti-PP2 kappa antibody

STJ95197 200 µl
EUR 236.4
Description: Rabbit polyclonal to PP2Ckappa.

Kappa Light chain Antibody

E046588 100μg/100μl
EUR 255
Description: Available in various conjugation types.

Kappa Light Chain Antibody

GWB-2AA0D3 1 ml Ask for price

kappa Light chain Antibody

GWB-2C9AA4 10 ml Ask for price

While p53 activity is associated with the induction of apoptosis, stimulation of NF-kB has been shown to promote resistance to programmed cell death.

Megan

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