BACKGROUND quercetin (Que) reported to induce apoptosis of lung cancer cells. Src is closely related to the development of lung cancer is non-small cell (NSCLC) and can be affected by Que in macrophages. In this study, the interaction between Que and Src signaling in NSCLC cell NSCLC explored to explain the anti-function Que.
MATERIALS AND METHODS H CC827 NSCLC cell lines were subjected to administration of Que at different concentrations. Que influence on tumor cell proliferation was detected using MTT and colony formation test. Then the effect on the migration and invasion ability was assessed using the initial and Transwell test. At the molecular level, changes in Src / Fn14 / NF-kappaB signaling is determined using western blotting tests.
The role of Src in Que function further explored by inducing the expression of Src gene in NSCLC cells prior to administration Que. The test results were verified in vitro using a mouse model of NSCLC. RESULTS Que inhibits the proliferation and anchorage-independent cell growth in NSCLC. Additionally, Que delayed in closing a gap in the level of initial tests and a decrease in the number of cells in the membrane-penetrating Transwell test. At the molecular level, Que suppressed the expression of Src, which then inhibit Fn14 / NF-kappaB signaling.
In in vivo tests, Que inhibits the growth of solid tumors. After the excess of Src in NSCLC cells, the effect of anti-NSCLC NSCLC Que blocked by inducing the proliferation and metastasis, and by activating Fn14 / NF-kappaB signaling. In addition, the induced levels of Src promoted the growth and metastatic potential of solid tumors in mice. CONCLUSION Que anti-NSCLC given effect by inhibiting Src-mediated Fn14 / NF-kappaB pathway both in vitro and in vivo.
Baicalin Inhibits Cell Proliferation and Inflammatory Cytokines Induced by Tumor Necrosis Factor α (TNF-α) in Human Immortalized Keratinocytes (HaCaT) Human Keratinocytes by Inhibiting the STAT3/Nuclear Factor kappa B (NF-κB) Signaling Pathway.
Osteoarthritis Prevented in Mice by Verbascoside via Nuclear Factor kappa B (NF-kB) Downregulation Pathway.
BACKGROUND Cartilage degeneration for osteoarthritis (OA), the most adversely affects the quality of life by inhibiting the movement. This study investigated the verbascoside role in the protection of cartilage degeneration caused by osteoarthritis. MATERIALS AND METHODS enzyme-linked immunosorbent assay (ELISA) and western blot test is used to determine the secretion of inflammatory cytokines in serum and cartilage tissue, respectively.
RESULTS Treatment of the mice with excess verbascoside OA inhibiting interleukin (IL) -6, tumor necrosis factor (TNF) -alpha, and IL-1SS in serum and cartilage tissue.
P2X7R expression and matrix metalloproteinase (MMP) -13 is much higher in mice induced by OA. However, the administration reversed verbascoside OA-induced upregulation of P2X7R and MMP-13 expression in cartilage tissue. OA-mediated increase in substance P (SP) and prostaglandin E2 (PGE2) expression was also reduced in cartilage tissue by verbascoside treatment. Western blot assay revealed that treatment verbascoside IkappaBalpha markedly decreased activation of NF-kappaB and p65 in mice OA.
CONCLUSION So, verbascoside inhibits the secretion of inflammatory cytokines in rats OA by targeting P2X7R expression, the production of matrix metalloproteinases, PGE2 and downregulation of NF-kappaB signaling pathway. Therefore, verbascoside can be used as an effective agent for the treatment of osteoarthritis.
The Onco-suppressor p53 is a transcription factor that regulates a broad spectrum of genes involved in various cellular functions including apoptosis, cell cycle arrest, senescence, autophagy, DNA repair and angiogenesis. p53 and NF-kB generally has the opposite effect on the cancer cells.
Kappa
MBS370349-01mLConcentrate
MyBiosource
0.1mL(Concentrate)
EUR 200
Kappa
MBS370349-05mLConcentrate
MyBiosource
0.5mL(Concentrate)
EUR 295
Kappa
MBS370349-1mLConcentrate
MyBiosource
1mL(Concentrate)
EUR 380
Human Kappa (kappa) Light Chain Antibody
MBS568905-1mg
MyBiosource
1mg
EUR 485
Human Kappa (kappa) Light Chain Antibody
MBS568905-5x1mg
MyBiosource
5x1mg
EUR 1980
Kappa Light Chain (Kappa-IgLC) Antibody
abx411144-1mg
Abbexa
1 mg
EUR 661.2
Kappa Light Chain (Kappa-IgLC) Antibody
abx411421-1ml
Abbexa
1 ml
EUR 526.8
Kappa Light Chain (Kappa-IgLC) Antibody
abx411422-05mg
Abbexa
0.5 mg
EUR 794.4
Kappa Light Chain (Kappa-IgLC) Antibody
abx412052-1ml
Abbexa
1 ml
EUR 526.8
Kappa Light Chain (Kappa-IgLC) Antibody
abx413125-1mg
Abbexa
1 mg
EUR 994.8
Kappa Light Chain (Kappa-IgLC) Antibody
abx415783-1mg
Abbexa
1 mg
EUR 526.8
Kappa Light Chain (Kappa-IgLC) Antibody
abx415786-05mg
Abbexa
0.5 mg
EUR 477.6
Kappa Light Chain (Kappa-IgLC) Antibody
abx415797-1mg
Abbexa
1 mg
EUR 594
Kappa Light Chain (Kappa-IgLC) Antibody
abx415799-05mg
Abbexa
0.5 mg
EUR 477.6
Kappa Light Chain (Kappa-IgLC) Antibody
abx413125-200l
Abbexa
200 µl
EUR 712.5
Kappa Light Chain (Kappa-IgLC) Antibody
abx415783-200l
Abbexa
200 µl
EUR 325
Kappa Light Chain (Kappa-IgLC) Antibody
abx415786-200l
Abbexa
200 µl
EUR 400
Kappa Light Chain (Kappa-IgLC) Antibody
abx415797-200l
Abbexa
200 µl
EUR 450
Kappa Light Chain (Kappa-IgLC) Antibody
abx415799-200l
Abbexa
200 µl
EUR 300
Kappa Light Chain (Kappa-IgLC) Antibody
abx411144-200g
Abbexa
200 µg
EUR 437.5
Kappa Light Chain (Kappa-IgLC) Antibody
abx411422-100tests
Abbexa
100 tests
EUR 550
Kappa Light Chain (Kappa-IgLC) Antibody
abx412052-250g
Abbexa
250 µg
EUR 325
immunoglobulin Kappa (immunoglobulin kappa) Antibody
abx234295-100ug
Abbexa
100 ug
EUR 610.8
Human kappa casein (kappa-CN) ELISA Kit
MBS2615385-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 3040
Human kappa casein (kappa-CN) ELISA Kit
MBS2615385-48StripWells
MyBiosource
48-Strip-Wells
EUR 285
Human kappa casein (kappa-CN) ELISA Kit
MBS2615385-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 1670
Human kappa casein (kappa-CN) ELISA Kit
MBS2615385-96StripWells
MyBiosource
96-Strip-Wells
EUR 405
Kappa Light Chain (Kappa-IgLC) Antibody (AP)
abx411146-1ml
Abbexa
1 ml
EUR 678
Kappa Light Chain (Kappa-IgLC) Antibody (AP)
abx411216-1ml
Abbexa
1 ml
EUR 678
Kappa Light Chain (Kappa-IgLC) Antibody (PE)
abx415789-200l
Abbexa
200 µl
EUR 525
Kappa Light Chain (Kappa-IgLC) Antibody (PE)
abx411219-250g
Abbexa
250 µg
EUR 650
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx411147-1ml
Abbexa
1 ml
EUR 594
Kappa Light Chain (Kappa-IgLC) Antibody (RPE)
abx411150-025mg
Abbexa
0.25 mg
EUR 760.8
Kappa Light Chain (Kappa-IgLC) Antibody (RPE)
abx411219-05mg
Abbexa
0.5 mg
EUR 910.8
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx412923-05mg
Abbexa
0.5 mg
EUR 861.6
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx413129-01mg
Abbexa
0.1 mg
EUR 594
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx414986-05mg
Abbexa
0.5 mg
EUR 861.6
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx415785-1ml
Abbexa
1 ml
EUR 594
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx415788-1ml
Abbexa
1 ml
EUR 594
Kappa Light Chain (Kappa-IgLC) Antibody (RPE)
abx415789-05ml
Abbexa
0.5 ml
EUR 760.8
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx412923-200l
Abbexa
200 µl
EUR 662.5
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx413129-200l
Abbexa
200 µl
EUR 375
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx414986-200l
Abbexa
200 µl
EUR 600
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx415785-200l
Abbexa
200 µl
EUR 387.5
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx415788-200l
Abbexa
200 µl
EUR 387.5
Kappa Light Chain (Kappa-IgLC) Antibody (ALP)
abx411146-200g
Abbexa
200 µg
EUR 462.5
Kappa Light Chain (Kappa-IgLC) Antibody (HRP)
abx411147-200g
Abbexa
200 µg
EUR 425
Kappa Light Chain (Kappa-IgLC) Antibody (ALP)
abx411216-25g
Abbexa
25 µg
EUR 450
Kappa Light Chain (Kappa-IgLC) Antibody (FITC)
abx411143-05mg
Abbexa
0.5 mg
EUR 526.8
Kappa Light Chain (Kappa-IgLC) Antibody (FITC)
abx415784-1mg
Abbexa
1 mg
EUR 560.4
Human Kappa Light Chain (Kappa-IgLC) Protein
abx670245-1mg
Abbexa
1 mg
EUR 661.2
Human Kappa Light Chain (Kappa-IgLC) Protein
abx670246-1mg
Abbexa
1 mg
EUR 777.6
Kappa Light Chain (Kappa-IgLC) Antibody (FITC)
abx415784-200l
Abbexa
200 µl
EUR 362.5
Human Kappa Light Chain (Kappa-IgLC) Protein
abx670245-100g
Abbexa
100 µg
EUR 462.5
Human Kappa Light Chain (Kappa-IgLC) Protein
abx670246-100g
Abbexa
100 µg
EUR 562.5
Kappa Light Chain (Kappa-IgLC) Antibody (FITC)
abx411143-200g
Abbexa
200 µg
EUR 325
Kappa Light Chain (Kappa-IgLC) Antibody (TRITC)
abx411145-05mg
Abbexa
0.5 mg
EUR 744
Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)
abx411149-1mg
Abbexa
1 mg
EUR 727.2
Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)
abx411218-1mg
Abbexa
1 mg
EUR 594
Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)
abx415787-05mg
Abbexa
0.5 mg
EUR 560.4
Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)
abx415787-200l
Abbexa
200 µl
EUR 362.5
Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)
abx411149-200g
Abbexa
200 µg
EUR 487.5
Kappa Light Chain (Kappa-IgLC) Antibody (Biotin)
abx411218-50g
Abbexa
50 µg
EUR 387.5
Mouse Immunoglobulin M Kappa (IgM Kappa) Protein
abx670294-1mg
Abbexa
1 mg
EUR 760.8
Mouse Immunoglobulin E Kappa (IgE Kappa) Protein
abx670299-05mg
Abbexa
0.5 mg
EUR 727.2
Mouse Immunoglobulin M Kappa (IgM Kappa) Protein
abx670294-100g
Abbexa
100 µg
EUR 637.5
Mouse Immunoglobulin E Kappa (IgE Kappa) Protein
abx670299-100g
Abbexa
100 µg
EUR 512.5
Human Immunoglobulin G1 Kappa (IgG1 Kappa) Protein
abx670165-1mg
Abbexa
1 mg
EUR 560.4
Human Immunoglobulin G2 Kappa (IgG2 Kappa) Protein
abx670166-1mg
Abbexa
1 mg
EUR 627.6
Mouse Immunoglobulin G1 Kappa (IgG1 Kappa) Protein
abx670291-1mg
Abbexa
1 mg
EUR 760.8
Mouse Immunoglobulin G1 Kappa (IgG1 Kappa) Protein
abx670291-100g
Abbexa
100 µg
EUR 700
Ig Kappa
ant-202
ProSpec Tany
0.5mg
EUR 275
Description: Mouse Anti Human Ig Kappa Light Chain
Mouse Immunoglobulin G2a Kappa (IgG2a Kappa) Protein
abx670292-1mg
Abbexa
1 mg
EUR 760.8
Mouse Immunoglobulin G2b Kappa (IgG2b Kappa) Protein
abx670293-1mg
Abbexa
1 mg
EUR 760.8
Mouse Immunoglobulin G2a Kappa (IgG2a Kappa) Protein
abx670292-100g
Abbexa
100 µg
EUR 612.5
Mouse Immunoglobulin G2b Kappa (IgG2b Kappa) Protein
abx670293-50g
Abbexa
50 µg
EUR 587.5
KAPPA/CD19
KAPPAF219PE1-50T
ImmunoStep
50 test
EUR 420.36
KAPPA/CD19
KF319PE4-50T
ImmunoStep
50 test
EUR 559.2
Human Kappa Light Chain (Kappa-IgLC) ELISA Kit
abx055741-96tests
Abbexa
96 tests
EUR 801.6
Kappa (NFKB1)
MO47031
Neuromics
100 ul
EUR 418.8
IgG2b, Kappa
MBS654957-5x1mg
MyBiosource
5x1mg
EUR 4310
IgG2b, Kappa
MBS655069-5x1mg
MyBiosource
5x1mg
EUR 3225
Kappa (NFKB1)
MBS556130-5x01mL
MyBiosource
5x0.1mL
EUR 1895
Rat Anti-Mouse Kappa (kappa chain)-UNLB Antibody
MBS4152264-01mg
MyBiosource
0.1mg
EUR 485
Rat Anti-Mouse Kappa (kappa chain)-UNLB Antibody
MBS4152264-5x01mg
MyBiosource
5x0.1mg
EUR 1930
Mouse Anti-Rat Kappa (kappa chain)-UNLB Antibody
MBS4152324-01mg
MyBiosource
0.1mg
EUR 650
Mouse Anti-Rat Kappa (kappa chain)-UNLB Antibody
MBS4152324-5x01mg
MyBiosource
5x0.1mg
EUR 2660
Casein kappa
E8ER1905-46
EnoGene
100ul
EUR 275
Description: Available in various conjugation types.
KAPPA/LAMBDA
KAPPAF2LAMBDAPE-50T
ImmunoStep
50 test
EUR 496.8
LAMBDA/KAPPA
LF2KPE3-50T
ImmunoStep
50 test
EUR 738.6
Casein kappa
MBS8577241-01mLAF405L
MyBiosource
0.1mL(AF405L)
EUR 565
Casein kappa
MBS8577241-01mLAF405S
MyBiosource
0.1mL(AF405S)
EUR 565
Casein kappa
MBS8577241-01mLAF610
MyBiosource
0.1mL(AF610)
EUR 565
Casein kappa
MBS8577241-01mLAF635
MyBiosource
0.1mL(AF635)
EUR 565
Kappa Light Chain (Kappa-IgLC) Antibody (Texas Red)
abx411148-05mg
Abbexa
0.5 mg
EUR 744
Kappa Light Chain (Kappa-IgLC) Antibody (Texas Red)
abx411217-1mg
Abbexa
1 mg
EUR 560.4
Kappa Light Chain (Kappa-IgLC) Antibody (Texas Red)
abx411217-500g
Abbexa
500 µg
EUR 362.5
Mouse Anti-Human Kappa (kappa chain)-UNLB Antibody
MBS4152443-01mg
MyBiosource
0.1mg
EUR 485
Mouse Anti-Human Kappa (kappa chain)-UNLB Antibody
MBS4152443-5x01mg
MyBiosource
5x0.1mg
EUR 1930
NFkB p52 (NF Kappa B, Nuclear Factor kappa B)
MBS621489-01mL
MyBiosource
0.1mL
EUR 760
NFkB p52 (NF Kappa B, Nuclear Factor kappa B)
MBS621489-5x01mL
MyBiosource
5x0.1mL
EUR 3270
Kappa-casein
AP81249
SAB
1mg
EUR 2640
Kappa-casein
AP81342
SAB
1mg
EUR 2640
Kappa-casein
AP81343
SAB
1mg
EUR 2640
Kappa-casein
AP81456
SAB
1mg
EUR 2640
Kappa-casein
AP81505
SAB
1mg
EUR 2640
Kappa Antibody
P1045-01m
SAB
0.1m
EUR 163.2
Kappa Antibody
P1045-1ml
SAB
1ml
EUR 337.2
Kappa Antibody
MBS9436898-INQUIRE
MyBiosource
INQUIRE
Ask for price
Immunoglobulin Kappa Light Chain (Ig kappa LC) Antibody
abx137077-01mg
Abbexa
0.1 mg
EUR 487.5
Carrageenan, Kappa
40300044-1
Glycomatrix
100 g
EUR 35.99
Carrageenan, Kappa
40300044-2
Glycomatrix
500 g
EUR 146.28
Human Kappa Immunoglobulin Light Chain, Kappa-IgLC (kappaFLC) ELISA Kit
MBS3801059-10x96StripWells
MyBiosource
10x96-Strip-Wells
EUR 6725
Human Kappa Immunoglobulin Light Chain, Kappa-IgLC (kappaFLC) ELISA Kit
MBS3801059-48StripWells
MyBiosource
48-Strip-Wells
EUR 550
Human Kappa Immunoglobulin Light Chain, Kappa-IgLC (kappaFLC) ELISA Kit
MBS3801059-5x96StripWells
MyBiosource
5x96-Strip-Wells
EUR 3420
Human Kappa Immunoglobulin Light Chain, Kappa-IgLC (kappaFLC) ELISA Kit
MBS3801059-96StripWells
MyBiosource
96-Strip-Wells
EUR 765
kappa-Bifenthrin
T24244-10mg
TargetMol Chemicals
10mg
Ask for price
Description: kappa-Bifenthrin
kappa-Bifenthrin
T24244-1mg
TargetMol Chemicals
1mg
Ask for price
Description: kappa-Bifenthrin
kappa-Bifenthrin
T24244-50mg
TargetMol Chemicals
50mg
Ask for price
Description: kappa-Bifenthrin
kappa-Bifenthrin
T24244-5mg
TargetMol Chemicals
5mg
Ask for price
Description: kappa-Bifenthrin
kappa-Bifenthrin
MBS5773994-5mg
MyBiosource
5(mg
EUR 915
kappa-Bifenthrin
MBS5773994-5x5mg
MyBiosource
5x5(mg
EUR 3970
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (AP)
MBS6489956-01mL
MyBiosource
0.1mL
EUR 1050
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (AP)
MBS6489956-5x01mL
MyBiosource
5x0.1mL
EUR 4575
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (PE)
MBS6489966-01mL
MyBiosource
0.1mL
EUR 1050
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (PE)
MBS6489966-5x01mL
MyBiosource
5x0.1mL
EUR 4575
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (APC)
MBS6489957-01mL
MyBiosource
0.1mL
EUR 1050
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (APC)
MBS6489957-5x01mL
MyBiosource
5x0.1mL
EUR 4575
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (HRP)
MBS6489960-01mL
MyBiosource
0.1mL
EUR 1050
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (HRP)
MBS6489960-5x01mL
MyBiosource
5x0.1mL
EUR 4575
kappa-Carrageenan
MBS5799138-5mg
MyBiosource
5(mg
EUR 915
kappa-Carrageenan
MBS5799138-5x5mg
MyBiosource
5x5(mg
EUR 3970
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (FITC)
MBS6489959-01mL
MyBiosource
0.1mL
EUR 1050
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (FITC)
MBS6489959-5x01mL
MyBiosource
5x0.1mL
EUR 4575
Rat Anti-Mouse Kappa (kappa chain)-AP Conjugate Antibody
MBS4152266-01mL
MyBiosource
0.1mL
EUR 605
Rat Anti-Mouse Kappa (kappa chain)-AP Conjugate Antibody
MBS4152266-5x01mL
MyBiosource
5x0.1mL
EUR 2475
Mouse Anti-Rat Kappa (kappa chain)-AP Conjugate Antibody
MBS4152326-01mL
MyBiosource
0.1mL
EUR 790
Mouse Anti-Rat Kappa (kappa chain)-AP Conjugate Antibody
MBS4152326-5x01mL
MyBiosource
5x0.1mL
EUR 3300
Kappa/Lambda Light Chain (Kappa/Lambda-IgLC) Antibody (HRP)
abx412924-05mg
Abbexa
0.5 mg
EUR 1028.4
Kappa/Lambda Light Chain (Kappa/Lambda-IgLC) Antibody (HRP)
abx412924-200l
Abbexa
200 µl
EUR 737.5
Rat Anti-Mouse Kappa (kappa chain)-HRP Conjugate Antibody
MBS4152267-01mL
MyBiosource
0.1mL
EUR 585
Rat Anti-Mouse Kappa (kappa chain)-HRP Conjugate Antibody
MBS4152267-5x01mL
MyBiosource
5x0.1mL
EUR 2370
Mouse Anti-Rat Kappa (kappa chain)-HRP Conjugate Antibody
MBS4152327-01mL
MyBiosource
0.1mL
EUR 720
Mouse Anti-Rat Kappa (kappa chain)-HRP Conjugate Antibody
MBS4152327-5x01mL
MyBiosource
5x0.1mL
EUR 2985
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (Biotin)
MBS6489958-01mL
MyBiosource
0.1mL
EUR 1050
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (Biotin)
MBS6489958-5x01mL
MyBiosource
5x0.1mL
EUR 4575
Opioid Receptor, kappa (OPRK1, Opioid Receptor, kappa 1, Kappa-type Opioid Receptor, KOR, KOR-1, OPRK)
MBS632940-01mg
MyBiosource
0.1mg
EUR 860
Opioid Receptor, kappa (OPRK1, Opioid Receptor, kappa 1, Kappa-type Opioid Receptor, KOR, KOR-1, OPRK)
MBS632940-5x01mg
MyBiosource
5x0.1mg
EUR 3725
Opioid Receptor, kappa (OPRK1, Opioid Receptor, kappa 1, Kappa-type Opioid Receptor, KOR, KOR-1, OPRK)
MBS628571-01mL
MyBiosource
0.1mL
EUR 510
While p53 activity is associated with the induction of apoptosis, stimulation of NF-kB has been shown to promote resistance to programmed cell death.
No Comment