NF-kB plays a major role in aetiopathogenesis of-inflammatory colitis. In this study, we evaluated the efficacy of green tea and polyphenols and their nanoformulation Tri-Nitro Benzene Sulfonic acid (TNBS) induced colitis in in-vivo systems (Rat) and the involvement of non-canonical and canonical NF-kB green line (platform di- silico) protection mediated tea. We use Wister rat model of TNBS-induced colitis.
Rats are grouped into eleven groups (six animals each) and administered vehicle (ethanol), TNBS, Epicatechin (EC), epigallocatechin (EGC), Epicatechin-gallate (ECG), epigallocatechin-gallate (EGCG), sulfasalazine, green tea, EGCG + sulfasalazine, nano-nano-EGCG and EGCG + sulfasalazine for 14 days after the induction of colitis. colonic tissue were evaluated for levels of malondialdehyde, myeloperoxidas e activity, catalase, reduced glutathione, glutathione peroxidase, IL-6, TNF-α, IL-1β, NF-kB and morphological and histopathological evidence of damage.
In part, in silico, molecular docking and EGCG dynamic simulation studies carried out on different targets in the NF-kB for a detailed evaluation of the role of non-canonical and canonical NF-KB pathway. In our study, EGCG reduces colon inflammation, oxidative stress markers, TNF-α, NF-kB, IL-1β and IL-6. Nano-EGCG + sulfasalazine is more efficacious when compared to EGCG + sulfasalazine.
In molecular docking and molecular dynamics simulation studies, EGCG showed good binding sites profile by IKK-beta inhibitors, IKK-alpha and NIK binding. Thus, it can be concluded that EGCG showed protective action in experimental colitis acting through both canonical and non-canonical NF-kB pathway. Nano-EGCG + sulfasalazine combination showed a better protection than the nano-EGCG alone. Communicated by H. Ramaswamy Sarma.
Efficacy of green tea, its polyphenols and nanoformulation in experimental colitis and the role of non-canonical and canonical nuclear factor kappa beta (NF-kB) pathway: a preclinical in-vivo and in-silico exploratory study
Retinoic acid-Platinum (II) Complexes [RT-Pt (II)] Protect Against Rheumatoid Arthritis in Mice via MEK / Nuclear Factor kappa B (NF-kB) Pathway Downregulation
BACKGROUND Rheumatoid arthritis (RA) is an inflammatory disorder that is present in approximately 1% of the world population. This study aims to determine the effect of retinoic acid-platinum (II) complex [RT-Pt (II)] in rheumatoid arthritis (RA) and to explore the mechanisms involved.
MATERIALS AND METHODS MH7A cell viability was determined by MTT assay and apoptosis was assessed using a FACSCalibur flow cytometry. RT-PCR and Western blot tests used to assess the mRNA and protein levels.
RESULTS Treatment of rheumatoid arthritis with RT-Pt (II) significantly reduced levels of IL-1SS, IL-6, IL-8, MMP-1 and MMP-13 in the synovial fluid of mice with a dose-dependent manner. Expression of iNOS and COX-2 mRNA and protein in rats with rheumatoid arthritis also significantly inhibited by treatment with RT-Pt (II). proliferation of TNF-alpha-induced cell MH7A was overcome by RT-Pt (II) treatment with a concentration-dependent manner.
In addition, RT-Pt (II) treatment induced apoptosis and causing cell cycle arrest in cells MH7A. Activation of MEK / NF-kappaB pathway were downregulated by RT-Pt (II) MH7A cell treatment. CONCLUSION In summary, this study showed that the RT-Pt (II) inhibit TNF-alpha-induced inflammatory response , suppress cell survival, and induces apoptosis in synovial cells of rheumatoid arthritis.
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (AP)
MBS6489956-01mL
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EUR 1050
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (AP)
MBS6489956-5x01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (PE)
MBS6489966-01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (PE)
MBS6489966-5x01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (APC)
MBS6489957-01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (APC)
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (HRP)
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MBS608702-02mg
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NFkB2 (Nuclear Factor kappa B, Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-cells 2)
MBS608693-01mg
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Nuclear Factor Kappa B (NFkB) Antibody
abx102274-1ml
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EUR 612.5
Nuclear Factor Kappa B (NFkB) Antibody
abx102274-200l
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Nuclear Factor Kappa B (NFkB) Antibody
abx102276-1ml
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EUR 712.5
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abx102276-200l
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Nuclear Factor Kappa B2 (NFkB2) Antibody
abx104779-100g
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Nuclear Factor Kappa B2 (NFkB2) Antibody
abx104779-20g
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Nuclear Factor Kappa B2 (NFkB2) Antibody
abx102466-1ml
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1 ml
EUR 712.5
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abx102466-200l
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abx102467-100l
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Nuclear Factor Kappa B2 (NFkB2) Antibody
abx102467-1ml
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1 ml
EUR 725
Nuclear Factor Kappa B2 (NFkB2) Antibody
abx102467-200l
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200 µl
EUR 337.5
Nuclear Factor Kappa B2 (NFKB2) Antibody
abx211537-100l
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abx211537-50l
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abx212536-100l
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abx212536-50l
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Nuclear Factor Kappa B2 (NFKB2) Antibody
abx334065-100g
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Nuclear Factor Kappa B2 (NFKB2) Antibody
abx334065-20g
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20 µg
EUR 162.5
Nuclear Factor Kappa B2 (NFKB2) Antibody
abx334065-50g
Abbexa
50 µg
EUR 250
Nuclear Factor Kappa B2 (NFKB2) Antibody
abx331414-100ul
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100 ul
EUR 510
Nuclear Factor Kappa B2 (NFKB2) Antibody
abx332017-100ul
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100 ul
EUR 510
Nuclear Factor Kappa B2 (NFKB2) Antibody
abx332100-100ul
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100 ul
EUR 510
Nuclear Factor Kappa B2 (NFKB2) Antibody
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abx329726-20g
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EUR 187.5
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abx329726-50g
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50 µg
EUR 250
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 405)
MBS6489961-01mL
MyBiosource
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EUR 1050
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 405)
MBS6489961-5x01mL
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EUR 4575
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 490)
MBS6489962-01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 490)
MBS6489962-5x01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 550)
MBS6489963-01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 550)
MBS6489963-5x01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 650)
MBS6489964-01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 650)
MBS6489964-5x01mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 750)
MBS6489965-01mL
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EUR 1050
NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 750)
MBS6489965-5x01mL
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EUR 4575
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2140209-001mg
MyBiosource
0.01mg
EUR 200
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2140209-005mg
MyBiosource
0.05mg
EUR 395
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2140209-01mg
MyBiosource
0.1mg
EUR 600
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2140209-02mg
MyBiosource
0.2mg
EUR 730
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2140209-05mg
MyBiosource
0.5mg
EUR 1410
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2031287-001mg
MyBiosource
0.01mg
EUR 180
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2031287-005mg
MyBiosource
0.05mg
EUR 335
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2031287-01mg
MyBiosource
0.1mg
EUR 515
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2031287-02mg
MyBiosource
0.2mg
EUR 630
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2031287-05mg
MyBiosource
0.5mg
EUR 1200
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029873-001mg
MyBiosource
0.01mg
EUR 170
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029873-005mg
MyBiosource
0.05mg
EUR 305
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029873-01mg
MyBiosource
0.1mg
EUR 470
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029873-02mg
MyBiosource
0.2mg
EUR 570
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029873-05mg
MyBiosource
0.5mg
EUR 1085
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029931-001mg
MyBiosource
0.01mg
EUR 160
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029931-005mg
MyBiosource
0.05mg
EUR 280
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029931-01mg
MyBiosource
0.1mg
EUR 405
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029931-02mg
MyBiosource
0.2mg
EUR 490
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029931-05mg
MyBiosource
0.5mg
EUR 930
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2010351-001mg
MyBiosource
0.01mg
EUR 155
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2010351-005mg
MyBiosource
0.05mg
EUR 260
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2010351-01mg
MyBiosource
0.1mg
EUR 375
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2010351-02mg
MyBiosource
0.2mg
EUR 460
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2010351-05mg
MyBiosource
0.5mg
EUR 860
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029099-001mg
MyBiosource
0.01mg
EUR 155
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029099-005mg
MyBiosource
0.05mg
EUR 260
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029099-01mg
MyBiosource
0.1mg
EUR 375
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029099-02mg
MyBiosource
0.2mg
EUR 460
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2029099-05mg
MyBiosource
0.5mg
EUR 860
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122871-001mg
MyBiosource
0.01mg
EUR 165
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122871-005mg
MyBiosource
0.05mg
EUR 285
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122871-01mg
MyBiosource
0.1mg
EUR 425
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122871-02mg
MyBiosource
0.2mg
EUR 515
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122871-05mg
MyBiosource
0.5mg
EUR 980
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122872-001mg
MyBiosource
0.01mg
EUR 160
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122872-005mg
MyBiosource
0.05mg
EUR 265
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122872-01mg
MyBiosource
0.1mg
EUR 385
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122872-02mg
MyBiosource
0.2mg
EUR 470
Recombinant Nuclear Factor Kappa B (NFkB)
MBS2122872-05mg
MyBiosource
0.5mg
EUR 885
Recombinant Nuclear Factor Kappa B (NFkB)
RPB824Bo01
Cloud-Clone
10ug
EUR 160
Recombinant Nuclear Factor Kappa B (NFkB)
RPB824Fi01
Cloud-Clone
10ug
EUR 235
Recombinant Nuclear Factor Kappa B (NFkB)
RPB824Ga01
Cloud-Clone
10ug
EUR 199
Recombinant Nuclear Factor Kappa B (NFkB)
4-RPB824Ga01
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Description: Recombinant Chicken Nuclear Factor Kappa B expressed in: E.coli
Recombinant Nuclear Factor Kappa B (NFkB)
RPB824Hu01
Cloud-Clone
10ug
EUR 140
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4-RPB824Hu01
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Description: Recombinant Human Nuclear Factor Kappa B expressed in: E.coli
Recombinant Nuclear Factor Kappa B (NFkB)
RPB824Hu02
Cloud-Clone
10ug
EUR 140
Recombinant Nuclear Factor Kappa B (NFkB)
4-RPB824Hu02
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Description: Recombinant Human Nuclear Factor Kappa B expressed in: E.coli
Recombinant Nuclear Factor Kappa B (NFkB)
RPB824Hu03
Cloud-Clone
10ug
EUR 144
Recombinant Nuclear Factor Kappa B (NFkB)
RPB824Mu01
Cloud-Clone
10ug
EUR 152
Recombinant Nuclear Factor Kappa B (NFkB)
4-RPB824Mu01
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Description: Recombinant Mouse Nuclear Factor Kappa B expressed in: E.coli
Recombinant Nuclear Factor Kappa B (NFkB)
RPB824Ra01
Cloud-Clone
10ug
EUR 179
Recombinant Nuclear Factor Kappa B (NFkB)
4-RPB824Ra01
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5 mg
10ug
50ug
100 ug
200 ug
500 ug
1 mg
Description: Recombinant Rat Nuclear Factor Kappa B expressed in: E.coli
In addition, RT-Pt (II) shows the effect by targeting the MEK pathway / NF-kappaB. Therefore, RT-Pt (II) can be used for the development of treatments for rheumatoid arthritis.
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