Efficacy of green tea, its polyphenols and nanoformulation in experimental colitis and the role of non-canonical and canonical nuclear factor kappa beta (NF-kB) pathway: a preclinical in-vivo and in-silico exploratory study

Efficacy of green tea, its polyphenols and nanoformulation in experimental colitis and the role of non-canonical and canonical nuclear factor kappa beta (NF-kB) pathway: a preclinical in-vivo and in-silico exploratory study

NF-kB plays a major role in aetiopathogenesis of-inflammatory colitis. In this study, we evaluated the efficacy of green tea and polyphenols and their nanoformulation Tri-Nitro Benzene Sulfonic acid (TNBS) induced colitis in in-vivo systems (Rat) and the involvement of non-canonical and canonical NF-kB green line (platform di- silico) protection mediated tea. We use Wister rat model of TNBS-induced colitis.

Rats are grouped into eleven groups (six animals each) and administered vehicle (ethanol), TNBS, Epicatechin (EC), epigallocatechin (EGC), Epicatechin-gallate (ECG), epigallocatechin-gallate (EGCG), sulfasalazine, green tea, EGCG + sulfasalazine, nano-nano-EGCG and EGCG + sulfasalazine for 14 days after the induction of colitis. colonic tissue were evaluated for levels of malondialdehyde, myeloperoxidase activity, catalase, reduced glutathione, glutathione peroxidase, IL-6, TNF-α, IL-1β, NF-kB and morphological and histopathological evidence of damage.

In part, in silico, molecular docking and EGCG dynamic simulation studies carried out on different targets in the NF-kB for a detailed evaluation of the role of non-canonical and canonical NF-KB pathway. In our study, EGCG reduces colon inflammation, oxidative stress markers, TNF-α, NF-kB, IL-1β and IL-6. Nano-EGCG + sulfasalazine is more efficacious when compared to EGCG + sulfasalazine.

In molecular docking and molecular dynamics simulation studies, EGCG showed good binding sites profile by IKK-beta inhibitors, IKK-alpha and NIK binding. Thus, it can be concluded that EGCG showed protective action in experimental colitis acting through both canonical and non-canonical NF-kB pathway. Nano-EGCG + sulfasalazine combination showed a better protection than the nano-EGCG alone. Communicated by H. Ramaswamy Sarma.

Efficacy of green tea, its polyphenols and nanoformulation in experimental colitis and the role of non-canonical and canonical nuclear factor kappa beta (NF-kB) pathway: a preclinical in-vivo and in-silico exploratory study
Efficacy of green tea, its polyphenols and nanoformulation in experimental colitis and the role of non-canonical and canonical nuclear factor kappa beta (NF-kB) pathway: a preclinical in-vivo and in-silico exploratory study

Retinoic acid-Platinum (II) Complexes [RT-Pt (II)] Protect Against Rheumatoid Arthritis in Mice via MEK / Nuclear Factor kappa B (NF-kB) Pathway Downregulation

BACKGROUND Rheumatoid arthritis (RA) is an inflammatory disorder that is present in approximately 1% of the world population. This study aims to determine the effect of retinoic acid-platinum (II) complex [RT-Pt (II)] in rheumatoid arthritis (RA) and to explore the mechanisms involved.

MATERIALS AND METHODS MH7A cell viability was determined by MTT assay and apoptosis was assessed using a FACSCalibur flow cytometry. RT-PCR and Western blot tests used to assess the mRNA and protein levels.

RESULTS Treatment of rheumatoid arthritis with RT-Pt (II) significantly reduced levels of IL-1SS, IL-6, IL-8, MMP-1 and MMP-13 in the synovial fluid of mice with a dose-dependent manner. Expression of iNOS and COX-2 mRNA and protein in rats with rheumatoid arthritis also significantly inhibited by treatment with RT-Pt (II). proliferation of TNF-alpha-induced cell MH7A was overcome by RT-Pt (II) treatment with a concentration-dependent manner.

In addition, RT-Pt (II) treatment induced apoptosis and causing cell cycle arrest in cells MH7A. Activation of MEK / NF-kappaB pathway were downregulated by RT-Pt (II) MH7A cell treatment. CONCLUSION In summary, this study showed that the RT-Pt (II) inhibit TNF-alpha-induced inflammatory response, suppress cell survival, and induces apoptosis in synovial cells of rheumatoid arthritis.

NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (AP)

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Rat Nuclear Factor kappa B (NFkB)

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Nuclear Factor Kappa B (NFkB) Antibody

abx128811-200l 200 µl
EUR 325

Nuclear Factor Kappa B (NFkB) Antibody

abx104767-100g 100 µg
EUR 775

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abx104767-20g 20 µg
EUR 275

Nuclear Factor Kappa B (NFkB) Antibody

abx104767-50g 50 µg
EUR 350

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abx102274-100l 100 µl
EUR 237.5

Nuclear Factor Kappa B (NFkB) Antibody

abx102274-1ml 1 ml
EUR 612.5

Nuclear Factor Kappa B (NFkB) Antibody

abx102274-200l 200 µl
EUR 300

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EUR 262.5

Nuclear Factor Kappa B (NFkB) Antibody

abx102276-1ml 1 ml
EUR 712.5

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abx332017-100ul 100 ul
EUR 510

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20-abx334065
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Nuclear Factor Kappa B2 (NFKB2) Antibody

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Nuclear Factor Kappa B2 (NFkB2) Antibody

abx173861-100l 100 µl
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EUR 250

Nuclear Factor Kappa B2 (NFKB2) Antibody

abx212536-100l 100 µl
EUR 350

Nuclear Factor Kappa B2 (NFKB2) Antibody

abx212536-50l 50 µl
EUR 250

Nuclear Factor Kappa B2 (NFKB2) Antibody

abx334065-100g 100 µg
EUR 362.5

Nuclear Factor Kappa B2 (NFKB2) Antibody

abx334065-20g 20 µg
EUR 162.5

Nuclear Factor Kappa B2 (NFKB2) Antibody

abx334065-50g 50 µg
EUR 250

Nuclear Factor Kappa B2 (NFKB2) Antibody

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Nuclear Factor Kappa B2 (NFKB2) Antibody

abx329726-20g 20 µg
EUR 187.5

Nuclear Factor Kappa B2 (NFKB2) Antibody

abx329726-50g 50 µg
EUR 250

Nuclear Factor Kappa B2 (NFKB2) Antibody

abx328264-100g 100 µg
EUR 250

Nuclear Factor Kappa B2 (NFKB2) Antibody

abx328264-50g 50 µg
EUR 187.5

Nuclear Factor Kappa B2 (NFkB2) Antibody

abx104779-100g 100 µg
EUR 775

Nuclear Factor Kappa B2 (NFkB2) Antibody

abx104779-20g 20 µg
EUR 275

Nuclear Factor Kappa B2 (NFkB2) Antibody

abx104779-50g 50 µg
EUR 350

Nuclear Factor Kappa B2 (NFkB2) Antibody

abx102466-100l 100 µl
EUR 262.5

Nuclear Factor Kappa B2 (NFkB2) Antibody

abx102466-1ml 1 ml
EUR 712.5

Nuclear Factor Kappa B2 (NFkB2) Antibody

abx102466-200l 200 µl
EUR 325

Nuclear Factor Kappa B2 (NFkB2) Antibody

abx102467-100l 100 µl
EUR 262.5

Nuclear Factor Kappa B2 (NFkB2) Antibody

abx102467-1ml 1 ml
EUR 725

Nuclear Factor Kappa B2 (NFkB2) Antibody

abx102467-200l 200 µl
EUR 337.5

NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 405)

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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 405)

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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 490)

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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 490)

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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 550)

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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 550)

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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 650)

MBS6489964-01mL 0.1mL
EUR 1050

NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 650)

MBS6489964-5x01mL 5x0.1mL
EUR 4575

NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 750)

MBS6489965-01mL 0.1mL
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NFkB p52 (NF Kappa B, Nuclear Factor kappa B) (MaxLight 750)

MBS6489965-5x01mL 5x0.1mL
EUR 4575

Recombinant Nuclear Factor Kappa B (NFkB)

RPU56201-100ug 100ug
EUR 633.6

Recombinant Nuclear Factor Kappa B (NFkB)

RPU56201-1mg 1mg
EUR 2496

Recombinant Nuclear Factor Kappa B (NFkB)

RPU56201-50ug 50ug
EUR 384

Recombinant Nuclear Factor Kappa B (NFkB)

RPU57796-100ug 100ug
EUR 483.9

Recombinant Nuclear Factor Kappa B (NFkB)

RPU57796-1mg 1mg
EUR 2246.4

Recombinant Nuclear Factor Kappa B (NFkB)

RPU57796-50ug 50ug
EUR 396

Recombinant Nuclear Factor Kappa B (NFkB)

RPU51165-100ug 100ug
EUR 468.6

Recombinant Nuclear Factor Kappa B (NFkB)

RPU51165-1mg 1mg
EUR 2074.8

Recombinant Nuclear Factor Kappa B (NFkB)

RPU51165-50ug 50ug
EUR 376.2

Recombinant Nuclear Factor Kappa B (NFkB)

RPU53408-100ug 100ug
EUR 552.2

Recombinant Nuclear Factor Kappa B (NFkB)

RPU53408-1mg 1mg
EUR 2446.6

Recombinant Nuclear Factor Kappa B (NFkB)

RPU53408-50ug 50ug
EUR 443.3

Recombinant Nuclear Factor Kappa B (NFkB)

RPU54571-100ug 100ug
EUR 613.8

Recombinant Nuclear Factor Kappa B (NFkB)

RPU54571-1mg 1mg
EUR 2719.6

Recombinant Nuclear Factor Kappa B (NFkB)

RPU54571-50ug 50ug
EUR 492.8

Recombinant Nuclear Factor Kappa B (NFkB)

RPB824Bo01 10ug
EUR 160

Recombinant Nuclear Factor Kappa B (NFkB)

RPB824Fi01 10ug
EUR 235

Recombinant Nuclear Factor Kappa B (NFkB)

RPB824Ga01 10ug
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Recombinant Nuclear Factor Kappa B (NFkB)

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Description: Recombinant Chicken Nuclear Factor Kappa B expressed in: E.coli

Recombinant Nuclear Factor Kappa B (NFkB)

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Description: Recombinant Human Nuclear Factor Kappa B expressed in: E.coli

Recombinant Nuclear Factor Kappa B (NFkB)

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Description: Recombinant Human Nuclear Factor Kappa B expressed in: E.coli

Recombinant Nuclear Factor Kappa B (NFkB)

RPB824Hu03 10ug
EUR 144

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Description: Recombinant Mouse Nuclear Factor Kappa B expressed in: E.coli

Recombinant Nuclear Factor Kappa B (NFkB)

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Description: Recombinant Rat Nuclear Factor Kappa B expressed in: E.coli

Recombinant Nuclear Factor Kappa B (NFkB)

RPU50627-100ug 100ug
EUR 431.2

Recombinant Nuclear Factor Kappa B (NFkB)

RPU50627-1mg 1mg
EUR 1911

Recombinant Nuclear Factor Kappa B (NFkB)

RPU50627-50ug 50ug
EUR 346.5

Recombinant Nuclear Factor Kappa B (NFkB)

RPU43097-100ug 100ug
EUR 431.2

Recombinant Nuclear Factor Kappa B (NFkB)

RPU43097-1mg 1mg
EUR 1911

Recombinant Nuclear Factor Kappa B (NFkB)

RPU43097-50ug 50ug
EUR 346.5

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029873-001mg 0.01mg
EUR 170

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029873-005mg 0.05mg
EUR 305

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029873-01mg 0.1mg
EUR 470

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029873-02mg 0.2mg
EUR 570

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029873-05mg 0.5mg
EUR 1085

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029931-001mg 0.01mg
EUR 160

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029931-005mg 0.05mg
EUR 280

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029931-01mg 0.1mg
EUR 405

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029931-02mg 0.2mg
EUR 490

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029931-05mg 0.5mg
EUR 930

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029099-001mg 0.01mg
EUR 155

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029099-005mg 0.05mg
EUR 260

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029099-01mg 0.1mg
EUR 375

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029099-02mg 0.2mg
EUR 460

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2029099-05mg 0.5mg
EUR 860

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2031287-001mg 0.01mg
EUR 180

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2031287-005mg 0.05mg
EUR 335

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2031287-01mg 0.1mg
EUR 515

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2031287-02mg 0.2mg
EUR 630

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2031287-05mg 0.5mg
EUR 1200

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2010351-001mg 0.01mg
EUR 155

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2010351-005mg 0.05mg
EUR 260

Recombinant Nuclear Factor Kappa B (NFkB)

MBS2010351-01mg 0.1mg
EUR 375

In addition, RT-Pt (II) shows the effect by targeting the MEK pathway / NF-kappaB. Therefore, RT-Pt (II) can be used for the development of treatments for rheumatoid arthritis.

Megan

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Baicalin Inhibits Cell Proliferation and Inflammatory Cytokines Induced by Tumor Necrosis Factor α (TNF-α) in Human Immortalized Keratinocytes (HaCaT) Human Keratinocytes by Inhibiting the STAT3/Nuclear Factor kappa B (NF-κB) Signaling Pathway.

Baicalin Inhibits Cell Proliferation and Inflammatory Cytokines Induced by Tumor Necrosis Factor α (TNF-α) in Human Immortalized Keratinocytes (HaCaT) Human Keratinocytes by Inhibiting the STAT3/Nuclear Factor kappa B (NF-κB) Signaling Pathway.

17-Aminogeldanamycin Inhibits Constitutive Nuclear Factor-Kappa B (NF-κB) Activity in Patient-Derived Melanoma Cell Lines

17-Aminogeldanamycin Inhibits Constitutive Nuclear Factor-Kappa B (NF-κB) Activity in Patient-Derived Melanoma Cell Lines

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